B.Valeur - Molecular Fluorescence Princeples And Applications.pdf

(5748 KB) Pobierz
Molecular Fluorescence: Principles and Applications.
Bernard Valeur
>
2001 Wiley-VCH Verlag GmbH
ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic)
Bernard Valeur
Molecular Fluorescence
Principles and Applications
Molecular Fluorescence: Principles and Applications.
Bernard Valeur
>
2001 Wiley-VCH Verlag GmbH
ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic)
Related Titles from WILEY-VCH
Broekaert, J. A. C.
Analytical Atomic Spectrometry
with Flames and Plasmas
2001.
isbn
3-527-30146-1
G€nzler, H. and Williams, A.
u
Handbook of Analytical Techniques
2 Volumes
2001.
isbn
3-527-30165-8
Feringa, B. L.
Molecular Switches
2001.
isbn
3-527-29965-3
Zander, C.; Keller, R. A. and Enderlein, J.
Single-Molecule Detection in Solution.
Methods and Applications
2002.
isbn
3-527-40310-8
Molecular Fluorescence: Principles and Applications.
Bernard Valeur
>
2001 Wiley-VCH Verlag GmbH
ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic)
Bernard Valeur
Molecular Fluorescence
Principles and Applications
Weinheim – New York – Chichester – Brisbane – Singapore – Toronto
Molecular Fluorescence: Principles and Applications.
Bernard Valeur
>
2001 Wiley-VCH Verlag GmbH
ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic)
Prof. Dr. Bernard Valeur
´ ´
Laboratoire de Chimie Generale
´
Conservatoire National des Arts et Metiers
292 rue Saint-Martin
75141 Paris Cedex 03
France
Cover
The photograph was provided by Prof. R.
Clegg (University of Illinois, USA).
9
This book was carefully produced.
Nevertheless, author and publisher do not
warrant the information contained therein
to be free of errors. Readers are advised to
keep in mind that statements, data,
illustrations, procedural details or other
items may inadvertently be inaccurate.
Library of Congress Card No.: applied for
A catalogue record for this book is available
from the British Library.
Die Deutsche Bibliothek – CIP Cataloguing-
in-Publication-Data
A catalogue record for this publication is
available from Die Deutsche Bibliothek
(
WILEY-VCH Verlag GmbH, 69469
Weinheim (Federal Republic of Germany).
2002
All rights reserved (including those of
translation in other languages). No part of
this book may be reproduced in any form –
by photoprinting, microfilm, or any other
means – nor transmitted or translated into
machine language without written
permission from the publishers. Registered
names, trademarks, etc. used in this book,
even when not specifically marked as such,
are not to be considered unprotected by law.
Printed in the Federal Republic of Germany.
Printed on acid-free paper.
Typesetting
Asco Typesetters, Hongkong
Printing
betz-druck gmbh, Darm-stadt
¨
Bookbinding
J. Schaffer GmbH&Co. KG,
¨
Grunstadt
ISBN
3-527-29919-X
Molecular Fluorescence: Principles and Applications.
Bernard Valeur
>
2001 Wiley-VCH Verlag GmbH
ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic)
v
Contents
Preface
Prologue
1
xii
1
3
Introduction
1.1
1.2
1.3
1.4
1.5
1.6
1.7
2
What is luminescence?
3
A brief history of fluorescence and phosphorescence
5
Fluorescence and other de-excitation processes of excited molecules
8
Fluorescent probes
11
Molecular fluorescence as an analytical tool
15
Ultimate spatial and temporal resolution: femtoseconds, femtoliters,
femtomoles and single-molecule detection
16
Bibliography
18
Absorption of UV–visible light
20
2.1
2.2
2.3
2.4
2.5
3
Types of electronic transitions in polyatomic molecules
20
Probability of transitions. The Beer–Lambert Law. Oscillator
strength
23
Selection rules
30
The Franck–Condon principle
30
Bibliography
33
Characteristics of fluorescence emission
34
34
3.1
3.1.1
3.1.2
3.1.3
3.1.3.1
3.1.3.2
3.1.3.3
3.1.3.4
3.2
3.2.1
Radiative and non-radiative transitions between electronic states
Internal conversion
37
Fluorescence
37
Intersystem crossing and subsequent processes
38
Intersystem crossing
41
Phosphorescence versus non-radiative de-excitation
41
Delayed fluorescence
41
Triplet–triplet transitions
42
Lifetimes and quantum yields
42
Excited-state lifetimes
42

Plik z chomika:

Inne pliki z tego folderu:

Inne foldery tego chomika:

Zgłoś jeśli naruszono regulamin