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2
Science, Matter,
and Energy
Carrying Out a Controlled
Scientific Experiment
CORE CASE STUDY
One way in which scientists learn about how nature works is to
conduct a
controlled experiment.
To begin, scientists isolate
vari-
ables,
or factors that can change within a system or situation
being studied. An experiment involving
single-variable analysis
is
designed to isolate and study the effects of one variable at a
time.
To do such an experiment, scientists set up two groups. One
is an
experimental group
in which a chosen variable is changed
in a known way and the other is a
control group
in which the
chosen variable is not changed. With proper experimental de-
sign, any difference between the two groups should result from
the variable that was changed in the experimental group.
In the 1960s, botanist Frank H. Bormann, forest ecologist
Gene Likens, and their colleagues began carrying out a classic
controlled experiment. The goal was to compare the loss of water
and nutrients from an uncut forest ecosystem (the
control site
)
with one that was stripped of its trees (the
experimental site
).
They built V-shaped concrete dams across the creeks at
the bottoms of several forested valleys in the Hubbard Brook
Experimental Forest in New Hampshire (Figure 2-1). The dams
were anchored on impenetrable bedrock so all surface water
leaving each forested valley had to flow across a dam, where sci-
entists could measure its volume and dissolved nutrient content.
The first experiment measured the amounts of water and
dissolved plant nutrients that entered and left an undisturbed
forested area (the control site, Figure 2-1, left). These baseline
data showed that an undisturbed mature forest is very efficient
at storing water and retaining chemical nutrients in its soils.
The next experiment involved disturbing the system and
observing any changes that occurred. One winter, the investiga-
tors cut down all trees and shrubs in one valley (the experimen-
tal site), left them where they fell, and sprayed with herbicides
to prevent the regrowth of vegetation. Then they compared the
inflow and outflow of water and nutrients in this modified ex-
perimental site (Figure 2-1, right) with those in the control site
for 3 years.
With no plants to help absorb and retain water, runoff of
water in the deforested valley increased by 30–40%. As this ex-
cess water ran rapidly over the surface of the ground, it eroded
soil and carried dissolved nutrients out of the deforested site.
Overall, the loss of key nutrients from the experimental forest
was six to eight times that in the nearby undisturbed forest.
Figure 2-1
Controlled field
experiment to measure the effects
of deforestation on the loss of wa-
ter and soil nutrients from a forest.
V–notched dams were built into
the impenetrable bedrock at the
bottoms of several forested valleys
(left) so that all water and nutri-
ents flowing from each valley
could be collected and measured
for volume and mineral content.
Baseline data were collected
on the forested valley (left) that
acted as the control site. Then all
the trees in one valley (the experi-
mental site) were cut (right) and
the flows of water and soil nutri-
ents from this experimental valley
were measured for three years.
83376_03_ch02_p023-037.ctp 8/10/07 11:47 AM Page 24
Key Questions and Concepts
2-1
What is science?
CONCEPT 2-1
Scientists collect data and develop theories,
models, and laws about how nature works.
CONCEPT 2-4B
Whenever energy is changed from one form to
another, we end up with lower quality or less usable energy than
we started with (second law of thermodynamics).
2-5
How can we use matter and energy more
sustainably?
CONCEPT 2-5A
The processes of life must conform to the
law of conservation of matter and the two laws of thermo-
dynamics.
CONCEPT 2-5B
We can live more sustainably by using and
wasting less matter and energy, recycling and reusing most matter
resources, and controlling human population growth.
2-2
What is matter?
CONCEPT 2-2
Matter consists of elements and compounds,
which are in turn made up of atoms, ions, or molecules.
2-3
How can matter change?
CONCEPT 2-3
When matter undergoes a physical or chemical
change, no atoms are created or destroyed (the law of conservation
of matter).
2-4
What is energy and how can it change its form?
CONCEPT 2-4A
When energy is converted from one form to
another in a physical or chemical change, no energy is created or
destroyed (first law of thermodynamics).
Note:
Supplements 1, 2, 6, 7, and 18 can be used with this chapter.
Science is an adventure of the human spirit.
It is essentially an artistic enterprise,
stimulated largely by curiosity,
served largely by disciplined imagination,
and based largely on faith in the reasonableness, order,
and beauty of the universe.
WARREN WEAVER
What Is Science?
2-1
CONCEPT 2-1
Scientists collect data and develop theories, models, and laws about how
nature works.
Science is a Search for Order
in Nature
Have you ever seen an area in a forest where all the
trees were cut down? If so, you might wonder about the
effects of cutting down all those trees. You might won-
der how it affected the animals and people living in that
area and how it affected the land itself. That is ex
actly
what scientists Bormann and Likens (
Core Case
Study
) thought about when they designed their
experiment.
Such curiosity is what motivates scientists.
Science
is an endeavor to discover how nature works and to
use that knowledge to make predictions about what
is likely to happen in nature. It is based on the assump-
tion that events in the natural world follow orderly
cause and effect patterns that can be understood
through careful observation, measurements, experi-
mentation, and modeling. Figure 2-2 summarizes the
scientific process.
There is nothing mysterious about this process. You
use it all the time in making decisions. Here is an ex-
ample of applying the scientific process to an everyday
situation:
Observation:
You switch on your flashlight and
nothing happens.
Question:
Why didn’t the light come on?
Hypothesis:
Maybe the batteries are dead.
Test the hypothesis:
Put in new batteries and switch
on the flashlight.
Result:
Flashlight still does not work.
24
Links:
refers to the Core Case Study.
refers to the book’s sustainability theme.
indicates links to key concepts in earlier chapters.
83376_03_ch02_p023-037.ctp 8/10/07 11:47 AM Page 25
ter and soil nutrients from cutover forests as a
problem worth studying.
Identify a problem
•
Find out what is known about the problem.
Bormann and Likens searched the scientific litera-
ture to find out what was known about the reten-
tion and loss of water and soil nutrients in forests.
Find out what is known
about the problem
(literature search)
•
Ask a question to be investigated.
The scien-
tists asked: “How does clearing forested land affect
its ability to store water and retain soil nutrients?
Ask a question to be
investigated
•
Design an experiment to answer the question
and collect data.
To collect
data,
or information
needed to answer their questions, scientists often
conduct
experiments,
or procedures carried out
under controlled conditions to gather information
and test ideas. Bormann and Likens collected
and analyzed data on the water and soil nutrients
flowing from a patch of an undisturbed forest (Fig-
ure 2-1, left) and from a nearby patch of forest
which they had cleared of trees for their experi-
ment (Figure 2-1, right).
Perform an experiment
to answer the question
and collect data
Scientific law
Well-accepted
pattern in data
Analyze data
(check for patterns)
Propose an hypothesis
to explain data
•
Propose an hypothesis to explain the data.
Scientists suggest a
scientific hypothesis,
or pos-
sible explanation of what they observe in nature.
The data collected by Bormann and Likens showed
a decrease in the ability of a cleared forest to store
water and retain soil nutrients such as nitrogen.
They came up with the following hypothesis, or
tentative explanation for their data: When a forest
is cleared, it retains less water and loses large
quantities of its soil nutrients when water from
rain and melting snow flows across its exposed
soil.
Use hypothesis to make
testable predictions
Perform an experiment
to test predictions
Accept
hypothesis
Revise
hypothesis
Make testable
predictions
•
Make testable predictions.
Scientists use an
hypothesis to make testable predictions about what
should happen if the hypothesis is valid. They
often do this by making “If ...then” predictions.
Bormann and Likens predicted that
if
their original
hypothesis was valid for nitrogen,
then
a cleared
forest should also lose other soil nutrients such as
phosphorus.
Test
predictions
Scientific theory
Well-tested and
widely accepted
hypothesis
Figure 2-2
What scientists do.
The essence of science is this process
for testing ideas about how nature works. Scientists do not necessar-
ily follow the order of steps described here. For example, sometimes
a scientist might start by formulating an hypothesis to answer the
initial questions and then run experiments to test the hypothesis.
•
Test the predictions with further experi-
ments, models, or observations.
To test their
prediction, Bormann and Likens repeated their
controlled experiment and measured the phos-
phorus content of the soil. Another way to test
predictions is to develop a
model,
an approximate
representation or simulation of a system being
studied. Since Bormann and Likens performed
their experiments, scientists have developed in-
creasingly sophisticated mathematical and com-
puter models of how a forest system works. Data
from Bormann and Likens’s research and that of
other scientists can be fed into such models and
used to predict the loss of phosphorus and other
types of soil nutrients. These predictions can be
compared with the actual measured losses to test
the validity of such models.
New hypothesis:
Maybe the bulb is burned out.
Experiment:
Replace bulb with a new bulb, and
switch on flashlight.
Result:
Flashlight works.
Conclusion:
Second hypothesis is verified.
Here is a more formal outline of steps scientists of-
ten take in trying to understand nature, although not
always in the order listed:
•
Identify a problem.
Bormann and Likens
(
Core Case Study
) identified the loss of wa-
25
CONCEPT 2-1
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•
Accept or reject the hypothesis.
If their new
data do not support their hypotheses, scientists
come up with other testable explanations. This
process continues until there is general agreement
among scientists in the field being studied that a
particular hypothesis is the best explanation of the
data. After Bormann and Likens confirmed that
the soil in a cleared forest also loses phosphorus,
they measured losses of other soil nutrients,
which also supported their hypothesis. A well-
tested and widely accepted scientific hypothesis or
a group of related hypotheses is called a
scientific
theory.
Thus, Bormann and Likens and their col-
leagues developed a theory that trees and other
plants hold soil in place and help it to retain water
and nutrients needed by the plants for their
growth.
Three important features of any scientific process
are
skepticism, peer review
of results by other scientists,
and
reproducibility.
Scientists tend to be highly skeptical
of new data and hypotheses until they can be verified.
Peer review
happens when scientists report details of
the methods they used, the results of their experiments
and models, and the reasoning behind their hypotheses
for other scientists working in the same field (their
peers) to examine and criticize. Ideally, other scientists
repeat and analyze the work to see if the data can be
reproduced and whether the proposed hypothesis is
reasonable and useful.
For example, the results of the forest experim
ents
by Bormann and Likens (
Core Case Study
) were
submitted to other soil and forest experts for
their review before a respected scientific journal would
publish their results. Other scientists have repeated the
measurements of soil content in undisturbed and
cleared forests of the same type and also for different
types of forests. Their results have also been subjected
to peer review. In addition, computer models of forest
systems have been used to evaluate this problem, with
the results subjected to peer review. Scientific knowl-
edge advances because scientists continually question
measurements, make new measurements, and try to
come up with new and better hypotheses (Science
Focus, at right).
Another important outcome of science is a
scien-
tific,
or
natural, law:
a well-tested and widely ac-
cepted description of what we find happening over and
over in the same way in nature. An example is the
law
of gravity,
based on countless observations and meas-
urements of objects falling from different heights. Ac-
cording to this law, all objects fall to the earth’s surface
at predictable speeds.
A good way to summarize the most important out-
comes of science is to say that scientists collect data and
develop theories, models, and laws that describe and
explain how nature works (
Concept 2-1
). Scientists use
reasoning and critical thinking skills (pp. 2–4). But the
best scientists also use intuition, imagination, and cre-
ativity in asking important questions, developing hy-
potheses, and designing ways to test them. Scientist
Warren Weaver’s quotation found at the opening of
this chapter reflects this aspect of science.
The Results of Science Can Be
Tentative, Reliable, or Unreliable
A fundamental part of science is
testing.
Scientists insist
on testing their hypotheses, models, methods, and re-
sults over and over again to establish the reliability of
these scientific tools, and the resulting conclusions.
Media news reports often focus on disputes among
scientists over the validity of scientific data, hypothe-
ses, models, methods, or results. This reveals differ-
ences in the reliability of various scientific tools and
results. Simply put, some science is more reliable than
other science, depending on how carefully it has been
done and on how thoroughly the hypotheses, models,
methods, and results have been tested.
Sometimes, preliminary results that capture news
headlines are controversial because they have not been
widely tested and accepted by peer review. They are
not yet considered reliable, and can be thought of as
tentative science
or
frontier science.
Some of these
results will be validated and classified as reliable and
some will be discredited and classified as unreliable. At
the frontier stage, it is normal for scientists to disagree
about the meaning and accuracy of data and the valid-
ity of hypotheses and results. This is how scientific
knowledge advances.
By contrast,
reliable science
consists of data, hy-
potheses, theories, and laws that are widely accepted by
scientists who are considered experts in the field. The
results of reliable science are based on the self-correct-
ing process of testing, open peer review, reproducibility,
and debate. New evidence and better hypotheses (Sci-
ence Focus, at right) may discredit or alter tried and ac-
cepted views. But unless that happens, those views are
considered to be the results of reliable science.
Scientific hypotheses and results that are presented
as reliable without having undergone the rigors of peer
Scientific Theories and Laws
Are the Most Important Results
of Science
If an overwhelming body of observations and measure-
ments supports a scientific hypothesis, it becomes a sci-
entific theory.
Scientific theories are not to be taken lightly.
They have been tested widely, are supported by exten-
sive evidence, and are accepted by most scientists in a
particular field or related fields of study.
26
CHAPTER 2
Science, Matter, and Energy
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SCIENCE FOCUS
F
Easter Island: Some Revisions in a Popular Environmental Story
or years, the story of Easter Island
has been used in textbooks as an ex-
ample of how humans can seriously degrade
their own life-support system. It concerns a
civilization that once thrived and then disap-
peared from a small, isolated island in the
great expanse of the South Pacific, located
about 3,600 kilometers (2,200 miles) off the
coast of Chile.
Scientists used anthropological evidence
and scientific measurements to estimate the
ages of certain artifacts found on Easter
Island (also called Rapa Nui). They hypothe-
sized that about 2,900 years ago, Polynesians
used double-hulled, seagoing canoes to colo-
nize the island. The settlers probably found a
paradise with fertile soil that supported dense
and diverse forests and lush grasses. Accord-
ing to this hypothesis, the islanders thrived,
and their population increased to as many as
15,000 people.
Measurements made by scientists indi-
cated that over time the Polynesians began
living unsustainably by using the island’s for-
est and soil resources faster than they could
be renewed. When they had used up the
large trees, the islanders could no longer
build their traditional seagoing canoes for
fishing in deeper offshore waters, and no one
could escape the island by boat.
Without the once-great forests to absorb
and slowly release water, springs and streams
dried up, exposed soils were eroded, crop
yields plummeted, and famine struck. There
was no firewood for cooking or keeping
warm. According to the original hypothesis,
the population and the civilization collapsed
as rival clans fought one another for dwin-
dling food supplies and the island’s popula-
tion dropped sharply. By the late 1870s, only
about 100 native islanders were left.
But in 2006, anthropologist Terry L. Hunt
evaluated the accuracy of past measurements
and other evidence and carried out new
measurements to estimate the ages of various
artifacts. He used these data to formulate an
alternative hypothesis describing the human
tragedy on Easter Island.
Hunt came to several conclusions.
First,
the Polynesians arrived on the island about
800 years ago, not 2,900 years ago.
Second,
their population size probably never exceeded
3,000, contrary to the earlier estimate of up to
15,000.
Third,
the Polynesians did use the is-
land’s trees and other vegetation in an unsus-
tainable manner, and by 1722 visitors reported
that most of the island’s trees were gone.
But one question not answered by the
earlier hypothesis was, why did the trees
never grow back? Recent evidence and Hunt’s
new hypothesis suggest that rats (which came
along with the original settlers either as stow-
aways or as a source of protein for their long
ocean voyage) played a key role in the island’s
permanent deforestation. Over the years, the
rats multiplied rapidly into the millions and
devoured the seeds that would have regener-
ated the forests.
Another of Hunt’s conclusions was that
after 1722, the population of Polynesians on
the island dropped to about 100, mostly from
contact with European visitors and invaders.
These newcomers introduced fatal diseases,
killed off some of the islanders, and took large
numbers of them away to be sold as slaves.
This story is an excellent example of how
science works. The gathering of new scientific
data and reevaluation of older data led to a
revised hypothesis that challenged our think-
ing about the decline of civilization on Easter
Island. The tragedy may not be as clear an ex-
ample of ecological collapse caused mostly by
humans as was once thought. However, there
is evidence that other earlier civilizations did
suffer ecological collapse largely from unsus-
tainable use of soil, water, and other re-
sources, as described in Supplement 6 on
p. S31.
Critical Thinking
Does the new doubt about the original Easter
Island hypothesis mean that we should not be
concerned about our apparent and growing
unsustainable use of essential natural capital
on the island in space we call the earth?
Explain.
review, or that have been discarded as a result of peer
review, are considered to be
unreliable science.
Here
are some critical thinking questions you can use to un-
cover unreliable science:
•
• Are the conclusions of the research widely accepted
by other experts in this field?
If “yes” is the answer to each of these questions,
then the results can be classified as
reliable science.
Otherwise, the results may represent
tentative science
that needs further testing and evaluation, or they can
be classified as
unreliable science.
See Supplement 17 on
pp. S73–S80 on How to Analyze a Scientific Paper.
Was the experiment well designed? Did it invol
ve
enough testing? Did it involve a control
group? (
Core Case Study
).
•
Have the data supporting the proposed hypotheses
been verified? Have the results been reproduced by
other scientists?
•
Do the conclusions and hypotheses follow logically
from the data?
Science and Environmental Science
Have Some Limitations
Before we continue our study of environmental sci-
ence, we need to recognize some of its limitations, as
well as those of science in general. First, scientists can
disprove things but cannot prove anything absolutely
because there is always some degree of uncertainty in
scientific measurements, observations, and models.
•
Are there no better scientific explanations?
•
Are the investigators unbiased in their interpre-
tations of the results? Are they free of a hidden
agenda? Were they funded by an unbiased source?
•
Have the conclusions been verified by impartial
peer review?
27
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